Video 1.

Time-lapse top/bottom 1,000-pixel images of the local membrane environment surrounding the insulin receptor. The whole time-lapse data set of PEG-Nile-Red labeled CHO cells expressing the ACP insulin receptor showing the top/bottom 1,000-pixel images exhibiting biggest (red) ZNCC changes and smallest (blue) 1,000 pixels of ZNCC in each ZNCC image (Fig. 6 A). Video corresponds to Fig. 7. Arrowheads show areas intensely blebbing and membrane ruffling. Video was acquired at 1 frame/10 s for 540 s and played back at 7 frames/s.


A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins

Miwa Umebayashi, Satoko Takemoto, Luc Reymond, Mayya Sundukova, Ruud Hovius, Annalisa Bucci, Paul A. Heppenstall, Hideo Yokota, Kai Johnsson, and Howard Riezman

DOI: 10.1083/jcb.202206119
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