Reversible axon beading affects the axonal trafficking of mobile mitochondria. Rat hippocampal neurons cultured in an AoC device were transfected with Lifeact-GFP and TagRFP-mito. Mild mechanical stress was induced by injecting low-speed flow (20 µl/min, 180 s) into the central injury chamber. Dual-color time-lapse images were acquired using a spinning disc microscope during the flux, showing the dynamic morphological changes of both the beading axon and the mitochondria. The appearance of cyan boxes marks the duration of the flux. The boxed ROI is magnified in the right panels, with arrowheads indicating the mobile mitochondria (magenta). Bar = 10 µm (left) and 5 µm (right). Video display rate: 7 fps.
