Mild mechanical stress causes the deformation of axonal mitochondria. In the AoC device, rat hippocampal neurons co-expressing Lifeact-GFP and TagRFP-mito were subjected to flux-induced mechanical stress (20 μl/min, 180 s). Dual-color time-lapse images were acquired using a spinning disc microscope during the flux, showing the dynamic morphological changes of both the beading axon and the mitochondria. The appearance of cyan boxes marks the duration of the flux. The boxed ROI is amplified in the right panels, with arrowheads indicating the compressed mitochondria (magenta) within the axonal beads (green). Bar = 20 µm (left) and 3 µm (right). Video display rate: 7 fps.
