CAR microcluster tracking analysis—HA dimer on low HER2 bilayer. TIRF imaging of CAR-mEmerald is shown for HA dimeric CAR on bilayer loaded with 6.25 ng/well. Spots show position of microclusters used for tracking. Scale bar is 3 μm. Time resolution per frame is 2.4 s, and total video is 40 frames (93.6 s).