Video 3.

Time-lapse video showing the partial colocalization and the associated movement between GFP-CFS1 (green) and VPS23A-TagRFP (magenta) in Arabidopsis root epidermal cells. 5-d-old Arabidopsis seedlings co-expressing pUBQ::GFP-CFS1 and pVPS23A::VPS23A-TagRFP were incubated in 150 mM NaCl-containing 1/2 MS media for 1 h for autophagy induction before imaging. Total imaging time, 60 s. Interval, 1 s. Scale bar, 10 μm.


Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole

Jierui Zhao, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti, Juan Carlos De La Concepcion, Yixuan Chen, Sofia Petsangouraki, Azadeh Mohseni, Marta García-Leon, Marta Salas Gomez, Caterina Giannini, Dubois Gwennogan, Roksolana Kobylinska, Marion Clavel, Swen Schellmann, Yvon Jaillais, Jiri Friml, Byung-Ho Kang, and Yasin Dagdas

DOI: 10.1083/jcb.202203139
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