ADA in MEF. MEF cell expressing GFP-F-tractin (green) and mito-BFP (red), stimulated with 20 μM CCCP at time 00:00 (min:s). Confocal images acquired every 15 s at medial region 2–4 μm above basal surface. Corresponds to Fig. 1 A. Scale bars: 10 and 5 μm (inset). Time stamp: min:s.