Video 1.

Related to Fig. 8 A. RNA was recruited into NPM1/DCAF13 droplets. 10 μM HEX-labeled polyU RNA oligo was added from the edge of the NPM1/DCAF13 mixture. Droplets formed with 40 µM DCAF13-Sof and 10 µM NPM1. DCAF13-Sof was labeled with Alexa Fluor 405 and NPM1 with Alexa Fluor 488. Images were collected at 20 s/frame. Time reported as min:s, Scale bar, 10 μm.


T cell proliferation requires ribosomal maturation in nucleolar condensates dependent on DCAF13

Lina Zhou, Shuai Wang, Wei Hu, Xiaoqian Liu, Lingdong Xu, Bolu Tong, Tongtong Zhang, Zhonghui Xue, Yixin Guo, Jing Zhao, Linrong Lu, Hengyu Fan, Wenbin Qian, Jian Chen, Wei Chen, and Lie Wang

DOI: 10.1083/jcb.202201096
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