Confocal time-lapse microscopy of Cdc3p-mCherry cells (PC7365) expressing GFP-Cdc42pQ61L (PC7458) grown on SD-URA media. Cells were grown for 5 h at 30°C. Green refers to GFP-Cdc42p, and Cdc3p-mCherry (red) is a septin ring marker. Time interval, 10 min, at 1 frame/s intervals.