Lysine residues of Cdc42p that were required for turnover of a GTP-locked version of the protein. The yeast Cdc42p protein sequence was overlaid onto the structure of human Cdc42p using the Expasy web server SWISS-MODEL (https://swissmodel.expasy.org; Nassar et al., 1998). Blue refers to K5, K94, and K96, and magenta to the effector-binding loop. Video displays at 25 frames/s intervals.