RapR-Shp2 activation and targeting to Gab1. HeLa cell coexpressing R32L RapR-Shp2-Cerulean-flag and FRB-GFP-Gab1 (Y628F/Y660F) were stained with CellMask Deep Red membrane marker dye and imaged live. CellMask Deep Red was imaged for 4.5 h every 2 min. Rapamycin (1 µM) was added at 90 min (indicated by appearance of “Rapamycin” sign).
