An integrin β3 molecule undergoing hop diffusion in the bulk basal PM entered the channel of the archipelago of FA-protein islands in an FA, and continued hop diffusion there. The FA-protein islands were identified by the simultaneously performed live-cell PALM of mEos3.2-paxillin (green regions; still image due to a 10-s data acquisition time = 1-ms integration time/frame × 10,000 frames). Ultrafast single-molecule imaging of a single SeTau647-Halo-integrin β3 molecule was performed at a 0.167-ms time resolution (magenta spot with a color-coded trajectory; replay, 50× slowed from real time) with a total observation period of 170 ms (1,024 frames). See Fig. 9 E and its legend.
