Video 5.

A single molecule of Setau647-ACP-integrin β3 (magenta spot with a yellow trajectory) diffused into an FA region and became temporarily immobilized (arrowhead) on an FA-protein island, as identified by the live-cell PALM of mEos3.2-paxillin (green), simultaneously performed with the single-molecule integrin tracking (4-ms resolution for 1 s; replay, 8.3× slowed from real time). See Fig. 9 A-a.


Ultrafast single-molecule imaging reveals focal adhesion nano-architecture and molecular dynamics

Takahiro K. Fujiwara, Taka A. Tsunoyama, Shinji Takeuchi, Ziya Kalay, Yosuke Nagai, Thomas Kalkbrenner, Yuri L. Nemoto, Limin H. Chen, Akihiro C.E. Shibata, Kokoro Iwasawa, Ken P. Ritchie, Kenichi G.N. Suzuki, and Akihiro Kusumi

DOI: 10.1083/jcb.202110162
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