A single molecule of Setau647-ACP-integrin β3 (magenta spot with a yellow trajectory) diffused into an FA region and became temporarily immobilized (arrowhead) on an FA-protein island, as identified by the live-cell PALM of mEos3.2-paxillin (green), simultaneously performed with the single-molecule integrin tracking (4-ms resolution for 1 s; replay, 8.3× slowed from real time). See Fig. 9 A-a.