Single TMR-Halo-TfR molecules diffusing in the basal PM of T24 cells and observed at a time resolution of 0.167 ms (a frame rate of 6 kHz) exhibited hop diffusion both outside and inside the FA. Movies on the left show larger view-fields: green areas represent FAs marked by mGFP-paxillin; magenta spots represent single TfR molecules. The regions in the yellow squares are enlarged in the movies on the right, showing single-molecule TfR movements outside (top) and inside (bottom) the FA. Within the FA region, the compartment area size is smaller by a factor of ≈2 and the dwell lifetime within a compartment is ≈1.5× longer, as compared with those outside the FA region. Total observation period of 1,500 frames = 250 ms. Replayed at a 50×-slowed rate. Refer to Fig. 8.
