Single Cy3 molecules covalently linked to the glass surface, observed at 10 kHz (every 0.1 ms; observation period of 1,500 frames = 150 ms; Replay, 167×-slowed from real time) using oblique-angle illumination at 23 µW/µm2. a, b, and c represent the fluorescent spots corresponding to those in Fig. 2, A and B. The on-periods including short off-periods of up to three frames are highlighted by the orange circle, and the off-periods are highlighted by the cyan circle.
