Ivy1 localization relative to vacuoles in vps4∆ cells by LLSM analysis after 3D deconvolution. Related to Fig. 3 C. vps4∆ cells expressing mGFP-tagged Ivy1 with CMAC-stained vacuoles were imaged for 1 min. The video shows 3D renderings (maximum-intensity projection mode in Imaris) of a deconvolved 3D time-lapse stack (see Materials and methods for details). The video was recorded at 8 frames/s; the original images were taken at 1.1-s time intervals.
