Reconstitution of more robust branching MT nucleation using recombinant complete augmin–γ-TuRC complex phosphorylated by CDK1 and PLK1. Branching MT nucleation in the presence of recombinant complete augmin–γ-TuRC complex (10 nM augmin; 1.0 nM γ-TuRC) copurified from nocodazole-arrested mitotic HEK293T cells expressing augmin-mCherry-Strep and γ-TuRC-GFP, together with untagged CDK1/cyclin B and HA-PLK1. Images were collected with a TIRF microscope at 4-s intervals. Video is sped up 160 times (40 frames/s). Time is shown in the format min:s. Scale bars, 5 μm.
