A closer inspection of augmin and γ-TuRC during the process of branching nucleation. Branching MT nucleation in the presence of native augmin–γ-TuRC complex (7.5 nM augmin; 0.15 nM γ-TuRC) purified from nocodazole-arrested mitotic HAUS8-GFP-Strep/GCP2-SNAP double knock-in HeLa cells. Images were collected with a TIRF microscope at 4-s intervals. Video is sped up 160 times (40 frames/s). Time is shown in the format min:s. Scale bars, 5 μm.
