Optogenetic pyroptosis induction in 3D organotypic cell cultures. Time-lapse fluorescence confocal microscopy of Caco-2 cells grown in 3D cultures (spheres) in Matrigel and locally stimulated with the blue light at 5 min. Inset (left) shows a stimulated cell displaying membrane blebbing and swelling, gaining DRAQ7 signal (blue) and being extruded from the sphere wall into the lumen. Scale bar, 50 µm.
