S100 and ChR2-YFP OptoNeurons in coculture spheres. 3D z-stack of Astrostim cells expressing S100. Astrostim cells were cocultured with OptoNeurons (ChR2-YFP) at a 1:500 ratio and treated with CNO for 2 d. See Fig. 3 D. Frame rate: 20 fps.
Caroline Cvetkovic, Rajan Patel, Arya Shetty, Matthew K. Hogan, Morgan Anderson, Nupur Basu, Samira Aghlara-Fotovat, Srivathsan Ramesh, Debosmita Sardar, Omid Veiseh, Michael E. Ward, Benjamin Deneen, Philip J. Horner, and Robert Krencik