Evaluation of the specificity of engineered Rok kinase. Dorsal closure was used as a model to test whether engineered Rok can activate other GFP-fusion proteins, which are not a direct target of Rok, and cause any obvious developmental defects. Please note an enhanced GFP signal in the engrailed domain, suggesting N-Rok::vhhGFP4Vi binding to GFP. Control (left): cadherin (shg)::GFP/+. Synthetic Rok (right): cadherin::GFP/UAS_N-Rok::vhhGFP4Vi. Imaging at 10 min intervals, 40× objective.
