Complete time-series of ABINM in chicken NTs treated with DMSO and transfected with shVCL. Chick NTs electroporated at HH12 stage with shVCL plus mbGFP (green) and H2B-RFP (magenta) vectors to visualize the plasma membrane and the nucleus, respectively, and treated with DMSO. After 8–10 h of incubation, GFP transfected areas were dissected and sectioned at 250 μm thickness. Confocal images of the slices were acquired every 5 min for 10–14 h using the Zeiss LSM-780 microscope with a 40× objective. The blue line shows the trajectory of a recorded cell. Reproduced at 30 fps. Selected video stills are shown in Fig. 4 C.
