Video 2.

Polymerization of ScMreB5^WT and ScMreB5^E134A in S. pombe cells. Time-lapse series showing the polymerization of GFP-ScMreB5^WT and GFP- ScMreB5^E134A in S. pombe cells observed by epifluorescence microscopy. Panels shown in Fig. 5 A (WT and E134A) were obtained from this time series. Images were deconvolved using SoftWorx software and are maximum-intensity projections from Z-stacks of 0.2 µm acquired at 3-min intervals. Scale bar represents 5 µm, and the frame rate is 10 fps.

Filament organization of the bacterial actin MreB is dependent on the nucleotide state

Vani Pande, Nivedita Mitra, Saket Rahul Bagde, Ramanujam Srinivasan, and Pananghat Gayathri

DOI: 10.1083/jcb.202106092