Segments of dendrite (left, middle) or dendrite growth cone (right) from live primary cortical neurons derived from wild-type (WT) or EVL knockout (KO) mice at day in vitro 11 (D11) expressing mRuby2-LifeAct. Cultures were treated with 0.1% DMSO, 100 µM CK-666, or 50 µM Blebbistatin at 5 min, and imaged for 40 min following treatment (treatment period indicated by yellow square). Growth cone dynamics were monitored as a read-out for approximating the time to maximal inhibition of Arp2/3 (∼20 min) and myosin activity (∼15 min) in neurons. Scale bar = 10 µm. 15 s interval, 45 min duration, 10 fps. See also Fig. 8.
