Live primary wild-type mouse cortical neurons at day in vitro 11 (D11) expressing EVL-iLID and MIM-iRFP670, and CAAX-iLID or MITO-iLID as indicated. Neurons were photostimulated with 488 nm laser light for 5 min, and imaged for 5 min before, during, and after photostimulation (stimulation period indicated by blue circle in row 2). Row 1: Segment of dendrite with merge of EVL-iLID (cyan), MIM-iRFP670 (red), and brightfield to better display DF dynamics. Row 2: Merge of EVL-iLID (cyan), MIM-iRFP670 (red) only. Rows 3 and 4: Rainbow intensity-coded LUT of EVL-iLID (row 3) or MIM-iRFP670 (row 4). Red = highest intensity, purple = lowest intensity. Scale bar = 10 µm. 5 s interval, 15 min duration, 10 fps. See also Fig. 7.
