Live primary wild-type mouse cortical neurons at day in vitro 11 (D11) expressing mRuby2-LifeAct, together with mEmerald-EVL and/or MIM-iRFP670 as indicated. Left: Segment of dendrite with overlay of brightfield and intensity-coded LUT of mRuby2-LifeAct. Right, bottom: Rainbow intensity-coded LUT of indicated constructs with outline of LifeAct-positive borders. Red = highest intensity, purple = lowest intensity. Right (MIM + EVL only): Merge of mEmerald-EVL (cyan) and MIM-iRFP670 (red). Scale bar = 10 µm. 5 s interval, 5 min duration, 10 fps. See also Fig. 7 and Fig. S7.
