Live primary EVL knockout (KO) mouse cortical neurons at day in vitro 11 (D11) expressing iRFP670-LifeAct and indicated iLID constructs. Neurons were photostimulated with 488 nm laser light for 5 min, and imaged for 5 min before, during, and after photostimulation (stimulation period indicated by blue circle). Row 1: Segment of dendrite with merge of indicated EVL-iLID constructs (cyan) and LifeAct (magenta). Row 2: iLID localization constructs CAAX-iLID or MITO-iLID (images acquired only during stimulation). Row 3: Rainbow intensity-coded LUT of indicated EVL-iLID constructs with outline of LifeAct-positive borders. Red = highest intensity, purple = lowest intensity. Row 4: Intensity-coded LUT of iRFP670-LifeAct. Scale bar = 10 µm. 5 s interval, 15 min duration, 10 fps. See also Fig. 6 and Fig. S6.
