Live EVL knockout (KO) cortical neurons at day in vitro 11 (D11) expressing mRuby2-LifeAct and mEmerald alone, or mEmerald-tagged wild-type EVL or mutants of EVL as indicated. Phase 1: Segment of dendrite with merge of mEmerald (cyan) and LifeAct (magenta). Phase 2: Intensity-coded LUT of mRuby2-LifeAct. Phase 3: Rainbow intensity-coded LUT of mEmerald with outline of LifeAct-positive borders. Red = highest intensity, purple = lowest intensity. Scale bar = 10 µm. 5 s interval, 5 min duration, 10 fps. See also Fig. 5.
