Live wild-type and EVL knockout (KO) cortical neurons at day in vitro 12 (D12) expressing EGFP-LifeAct and PSD95-FingR-mRuby2. Row 1: Segment of dendrite with merge of PSD95 reporter (green) and LifeAct (magenta). Row 2: LifeAct fluorescence intensity presented with an inverted LUT to highlight actin dynamics. Row 3: PSD95 reporter fluorescence intensity presented with an inverted LUT. Scale bar = 10 µm. 30 s interval, 50 min duration, 10 fps. See also Fig. 4.
