Live primary cortical neurons derived from wild-type (WT) or EVL knockout (KO) mice at indicated days in vitro expressing mRuby2-LifeAct. Left: segment of dendrite with overlay of brightfield and intensity-coded LUT of mRuby2-LifeAct. Right: mRuby2-LifeAct alone. Scale bar = 10 µm. 5 s interval, 5 min duration, 10 fps. See also Fig. 4 and Fig. S5.