Live primary mouse cortical neurons at day in vitro 11 (D11) expressing mRuby2-LifeAct and EGFP (top row), EGFP-MENA (middle row), or EGFP-EVL (bottom row). Left: segment of dendrite with merge of EGFP (cyan) and LifeAct (magenta). Right: rainbow intensity-coded LUT of EGFP with outline of LifeAct-positive borders. Red = highest intensity, purple = lowest intensity. Scale bar = 10 µm. 5 s interval, 5 min duration, 10 fps. See also Fig. 2 and Fig. S3.