Video 3.

Time-lapse widefield fluorescent microscopy of entotic cell-in-cell structures in MCF10A cells expressing GFP-hLC3A treated ± SaliP (SaliPhe; 2.5 μM). Movie shows GFP-hLC3A recruitment to entotic vacuoles during inner cell death. GFP-hLC3A recruitment is prolonged in the presence of SaliP. GFP and DIC images acquired every 10 min for 20 h. Movie plays 8 frames/s; time, h:min. Related to Fig. 5 i.


V-ATPase is a universal regulator of LC3-associated phagocytosis and non-canonical autophagy

Kirsty M. Hooper, Elise Jacquin, Taoyingnan Li, Jonathan M. Goodwin, John H. Brumell, Joanne Durgan, and Oliver Florey

DOI: 10.1083/jcb.202105112
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