Time-lapse confocal imaging of gastrula-stage Xenopus embryo treated with 20 µM BAPTA-AM + 100 µM 2-APB for 1 h. Video shows severe, long-lasting ZO-1 breaks (white arrows) and reduced activation of Rho flares (yellow arrowheads) in the absence of calcium flashes (GCaMP6m). All channels shown in FIRE LUT. Time interval, 5 s; video frame rate, 50 fps. Related to Fig. 4 C.
