Time-lapse confocal imaging of gastrula-stage Xenopus embryo shows local and sustained increase in calcium (R-GECO1, green) and F-actin accumulation (Lifeact-GFP, magenta) at the site of a laser-induced TJ breach. Note that the R-GECO1 intensity increases throughout the field of view only when 405-nm laser is turned on to induce laser injury. Time interval, 6.5 s; video frame rate, 10 fps. Related to Fig. 2 E and Fig. S1 G.
