Video 4.

Time-lapse confocal imaging shows the dynamic activation of Rho flares (mCherry-2xrGBD, magenta) at the site of ZO-1 loss (BFP-ZO-1, cyan) and calcium flashes (GCaMP6m, green). Time interval, 5 s; video frame rate, 20 fps. Related to Fig. 2, C and D.

Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling

Saranyaraajan Varadarajan, Shahana A. Chumki, Rachel E. Stephenson, Eileen R. Misterovich, Jessica L. Wu, Claire E. Dudley, Ivan S. Erofeev, Andrew B. Goryachev, and Ann L. Miller

DOI: 10.1083/jcb.202105107