Time-lapse confocal imaging of gastrula-stage Xenopus embryo showing leaks (FluoZin-3, orange LUT) in embryos expressing BFP-ZO-1 (FIRE LUT) and active Rho probe (mCherry-2xrGBD, FIRE LUT). First video shows the local increase of FluoZin-3 (white arrow) preceding the Rho flare (yellow arrowhead) at the site of ZO-1 decrease (white arrowhead) in vehicle (water) treated embryos. Second video shows repeated local increases of FluoZin-3 (green and white arrows) followed by short-duration Rho flare (yellow arrowhead) in embryos treated with 12.5 µM GsMTx4. Time interval, 21 s; video frame rate, 5 fps. Related to Fig. 7, D–E.
