Time-lapse confocal imaging of gastrula-stage Xenopus embryo showing that local calcium (BFP-C2, green) and active Rho (mCherry-2xrGBD, magenta) increase at the site of leaks (FluoZin-3, FIRE LUT). Video illustrates that the FluoZin-3 increase precedes the local calcium increase. Time interval, 21 s; video frame rate, 5 fps. Related to Fig. 1, A and B.
