Fate of mislocalized Frt1 in the presence of Msp1, but in the absence of Get3-AID*-9xMyc. Expression of 3xFLAG-mNG-Frt1 (green) was induced by addition of cyanamide in the presence of IAA. After shutoff of 3xFLAG-mNG-Frt1 expression, Get3-AID*-9xMyc was again depleted by addition of IAA for 30 min before addition of β-estradiol for Msp1 expression and then time-lapse imaging was recorded every 2 min. Single-plane image is shown. Mitochondria was labeled with Tom70-mCherry (red). Cell outlines (blue) are shown by a single DIC image. Scale bar, 5 μm.
