Fate of mislocalized Frt1 in the presence of Get3-AID*-9xMYC, but in the absence of Msp1. Expression of 3xFLAG-mNG-Frt1 (green) was induced by addition of cyanamide in the presence of IAA. After shutoff of 3xFLAG-mNG-Frt1 expression, Get3-AID*-9xMyc was re-expressed for 2 h, then time-lapse imaging was recorded every 2 min. Single-plane image is shown. Mitochondria were labeled with Tom70-mCherry (red). Cell outlines (blue) are shown by a single DIC image. Scale bar, 5 μm. Frame rate is 7 fps.
