Fate of mislocalized Frt1 in the presence of Msp1 and Get3-AID*-9xMyc. Expression of 3xFLAG-mNG-Frt1 (green) was induced by addition of cyanamide in the presence of IAA. After shutoff of 3xFLAG-mNG-Frt1 expression, Get3-AID*-9xMyc was re-expressed for 2 h, then the Msp1 expression was induced by addition of β-estradiol and time-lapse imaging was recorded every 2 min. Single-plane image is shown. The ER was labeled with Snd3-mCherry (red). Left, 3xFLAG-mNG-Frt1 (green); central, Snd3-mCherry (red); right, merge. Cell outlines (blue) are shown by a single DIC image. Scale bar, 5 μm. Frame rate is 7 fps.
