WASP forms puncta that are stationary relative to the substrate but undergo retrograde flux in the absence of integrin engagement (+EDTA; as in Fig. 1 E). Scale bar is 5 μm. Imaged with TIRF microscopy. Video is displayed at 30 fps.
Rachel M. Brunetti, Gabriele Kockelkoren, Preethi Raghavan, George R.R. Bell, Derek Britain, Natasha Puri, Sean R. Collins, Manuel D. Leonetti, Dimitrios Stamou, and Orion D. Weiner