Video 4.

Progranulin-RUSH trafficking from the ER in Surf4 KO cells. Confocal microscopy shows trafficking of progranulin-RUSH (magenta) from the ER in Surf4 KO cells beginning 6 min after biotin addition (images were acquired at 3-min intervals). eGFP-GalT labels the Golgi compartment (green). Video plays at 2 frames/s with time display in h:min format. Scale bar, 10 µm.

Efficient progranulin exit from the ER requires its interaction with prosaposin, a Surf4 cargo

Swathi Devireddy, and Shawn M. Ferguson

DOI: 10.1083/jcb.202104044