Response of individual neutrophil to LW. In support of Figs. 3 and S2. (A) Neutrophil in a Tg(mpx:Lifeact-Ruby) zebrafish larva in VF in 30 nM LTB4. LW occurs at 6:40 min (not visible in the video, location is indicated by angle). Time-lapse projections of Fig. 3 A correspond to 00:20, 07:40, 08:00, and 11:00 min, respectively, at video. Frame interval is 20 s. (B) Neutrophil in a Tg(mpx:Lifeact-Ruby) zebrafish larva in head. LW occurs at 5:00 min (not visible in the video, location is indicated by angle). Time-lapse projections of Fig. S2 C correspond to 4:30, 7, and 10 min, respectively, at video. Frame interval is 30 s. (A and B) White arrow indicates the neutrophil to observe. Red/green arrows indicate Lifeact at the front/rear. Frame rate is 5 fps. Scale bar = 20 μm.
