Formation of a multicellular cyst in vitro upon Rab35 silencing. Live-cell time-lapse microscopy documenting the formation of a multicellular cyst upon Rab35 silencing in differentiating hepatoblasts expressing LifeAct-EGFP. Images acquired in 10-min intervals. The video displays a 51-h time window at 12,000× normal speed. Scale bar: 10 µm.