Video 7.

Formation of a spherical lumen upon Rab35 silencing in vitro. Live-cell time-lapse microscopy showing the growth of a spherical lumen between two differentiating hepatoblasts expressing LifeAct-EGFP upon Rab35 silencing. Images acquired in 10-min intervals. The video displays a 29-h time window at 12,000× normal speed. Scale bar: 10 µm.

Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads

Lenka Belicova, Urska Repnik, Julien Delpierre, Elzbieta Gralinska, Sarah Seifert, José Ignacio Valenzuela, Hernán Andrés Morales-Navarrete, Christian Franke, Helin Räägel, Evgeniya Shcherbinina, Tatiana Prikazchikova, Victor Koteliansky, Martin Vingron, Yannis L. Kalaidzidis, Timofei Zatsepin, and Marino Zerial

DOI: 10.1083/jcb.202103003