Video 2.

Tomogram of MVB in a bro1Δ cell. bro1Δ cell has class E compartments, which are flattened stacks of endosomal membranes that generally lack internal vesicles. Cisternal class E compartment stacks are shown in different colors to differentiate individual membranes. The vacuolar limiting membrane is labeled red. Scale bar = 100 nm. Frame rate, 50 frames/s.


Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis

Chun-Che Tseng, Shirley Dean, Brian A. Davies, Ishara F. Azmi, Natalya Pashkova, Johanna A. Payne, Jennifer Staffenhagen, Matt West, Robert C. Piper, Greg Odorizzi, and David J. Katzmann

DOI: 10.1083/jcb.202102070
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