Video 1.

Tomogram of MVB in a bro1^ΔBOD cell. MVBs in a bro1^ΔBOD cell exhibit WT-like MVB morphology. This video shows nine MVBs that are adjacent to the vacuole (red mesh), while the limiting membrane of the MVB is colored yellow and the ILVs are in red. Intermediate BPs are highlighted in green. Scale bar = 100 nm. Frame rate, 50 frames/s.

Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis

Chun-Che Tseng, Shirley Dean, Brian A. Davies, Ishara F. Azmi, Natalya Pashkova, Johanna A. Payne, Jennifer Staffenhagen, Matt West, Robert C. Piper, Greg Odorizzi, and David J. Katzmann

DOI: 10.1083/jcb.202102070