Video 5.

FRAP analysis of MyD88-GFP (related to Fig. 5). This video shows an EL4 cell expressing MyD88-GFP in which a region of interest (red box) is photobleached. The video illustrates that Myddosomes show no fluorescence intensity recovery after photobleaching. Same cell as shown in Fig. 5 A. Scale bar, 5 µm.

MyD88 oligomer size functions as a physical threshold to trigger IL1R Myddosome signaling

Rafael Deliz-Aguirre, Fakun Cao, Fenja H.U. Gerpott, Nichanok Auevechanichkul, Mariam Chupanova, YeVin Mun, Elke Ziska, and Marcus J. Taylor

DOI: 10.1083/jcb.202012071