Video 4.

IRAK1 recruitment to clusters of MyD88 (related to Fig. 4). This video shows an EL4 cell expressing MyD88-GFP (left, green channel in merge) and IRAK1-mScarlet (middle, magenta channel in merge) interacting with an IL1β-functionalized supported lipid membrane. The video illustrates the nucleation of MyD88-GFP puncta and the recruitment of IRAK1-mScarlet. Same cell as shown in Fig. 4 B. Scale bar, 5 µm.

MyD88 oligomer size functions as a physical threshold to trigger IL1R Myddosome signaling

Rafael Deliz-Aguirre, Fakun Cao, Fenja H.U. Gerpott, Nichanok Auevechanichkul, Mariam Chupanova, YeVin Mun, Elke Ziska, and Marcus J. Taylor

DOI: 10.1083/jcb.202012071