Video 5.

Time-lapse images of dynamic astral microtubules in a mock-treated (DMSO) HU-arrested (S-phase) Tub3-only cell with microtubules labeled with GFP-Tub1 and their plus ends enhanced with Bik1-3GFP. Images were captured at 8-s intervals and represent composite maximum projections of 10 z-planes spaced 0.75 µm apart. Video plays at 10 fps and encompasses 9.3 min.


Tubulin isotypes optimize distinct spindle positioning mechanisms during yeast mitosis

Emmanuel T. Nsamba, Abesh Bera, Michael Costanzo, Charles Boone, and Mohan L. Gupta

DOI: 10.1083/jcb.202010155
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