Time-lapse imaging of mitotic live bovine zygote expressing EGFP-MAP4 (green) and H2B-mCherry (magenta) after mRNA injection at pronuclear stage. Time resolution, 2.5 min. Scale bar, 10 µm. Movie shows 30 frames/s. Recordings start at 2.5 min before NEBD of the leading PN (PN1). Movie shows distant dual spindle assembly in the context of the entire imaged volume of one zygote, also depicted in Fig. 3 C and S4, E and F.
